Contact an Account ManagerAmine derivatization with luminarins 1 and 2: separation by LC with peroxyoxalate chemiluminescence detection
Summary
Type: HPLC
Application #: 102300
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 390nm, emission 490nm
Conditions: eluent: acetonitrile - 5mM ammonium acetate (26:75, v/v)
flow rate 2ml/min
Authors: Tod M
Source: J Chromatogr 594 (1992) 386 - 391
Details
Legend: Fig. 3. Separation of the histamine luminarin derivative; left, blank, right
0.5nmol derivatized histamine (50pmol injected); numbers at peaks indicate
retention times.